Detecting Proliferating Cell Nuclear Antigen in Archival Rodent Tissues
نویسندگان
چکیده
منابع مشابه
Detecting proliferating cell nuclear antigen in archival rodent tissues.
The detection of proliferating cell nuclear antigen (PCNA), an endogenous cell replication marker, has lacked sensitivity in paraffin-embedded archival tissues fixed in formalin. An enhanced immunohistochemical procedure to detect PCNA has been successfully applied to rat and mouse tissues. Tissue sections are heated in a microwave oven in the presence of an antigen-retrieval solution of heavy-...
متن کاملAntisense Proliferating Cell Nuclear Antigen Oligonucleotides
We have used antisense phosphorothioate oligonucleotides to define the role played by proliferating cell nuclear antigen (PCNA) in neointimal accumulation of smooth muscle cells in a rat carotid artery injury model. The short-term extraluminal delivery of 250 nmol of antisense oligonucleotides, but not control oligonucleotides, immediately after arterial injury produces a 77% suppression of PCN...
متن کاملIndirect immunofluorescence studies of proliferating cell nuclear antigen in nucleoli of human tumor and normal tissues.
A proliferating cell nuclear antigen (PCNA) was identified with autoantibodies from a patient with systemic lupus erythematosus. Specific antibodies were purified by affinity chromatography in which Novikoff hepatoma nucleolar proteins were conjugated to Sepharose-4B. The purified anti-PCNA antibodies produced bright nucleolar fluorescence in tumor cells as shown by indirect immunofluorescence....
متن کاملRegulation of proliferating cell nuclear antigen ubiquitination in mammalian cells.
After exposure to DNA-damaging agents that block the progress of the replication fork, monoubiquitination of proliferating cell nuclear antigen (PCNA) mediates the switch from replicative to translesion synthesis DNA polymerases. We show that in human cells, PCNA is monoubiquitinated in response to methyl methanesulfonate and mitomycin C, as well as UV light, albeit with different kinetics, but...
متن کاملProliferating cell nuclear antigen expression in childhood acute leukemia.
Proliferating cell nuclear antigen (PCNA) is a 36-Kd nuclear protein, identified as the auxiliary protein of DNA polymerase delta, that is upregulated in activated proliferating cells from a variety of tissues and species, including human lymphocytes. We have examined by two-dimensional polyacrylamide gel electrophoresis the expression of PCNA in various subtypes of childhood acute leukemia and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Environmental Health Perspectives
سال: 1993
ISSN: 0091-6765
DOI: 10.2307/3431869